Corrigendum to “Non-thermal atmospheric-pressure plasma potentiates mesodermal differentiation of human induced pluripotent stem cells” [Heliyon 8(12) (December 2022) e12009]

In the original published version of this article, text “Immediately after plasma exposure, cells were washed with PBS and dissociated using Accutase® (Innovative Cell Technologies, Inc., San Diego, CA, USA), counted, 1–2 × 106 plated in a 10-cm non-coated dish. Cells cultured for four days EB differentiation medium followed by three-day culture consisted 80% DMEM (with glutamine non-essential amino acids) 20% FBS. On day 7, lysed total RNA was extracted RNAzol ® -RT (Molecular Research Center, Cincinnati, OH, USA). Absorbances at 260 280 nm measured NanoDrop™ ND-1000 spectrophotometer (Thermo Fisher Scientific Waltham, MA, USA) quantification. Using RTase (Takara Bio Otsu, Japan), cDNA synthesized qPCR conducted TaqMan hPSC Scorecard™ Panel (product no. A15871, Thermo Inc.) on StepOnePlus™ real-time machine (Applied Biosystems). PCR reaction gene analysis following manufacturer's protocol Analysis Software, Web-based system provided manufacturer. amplification started 20-s denaturation step 95 °C 40 cycles 1 s 60 20 s. The 70% DMEM, FBS, 10% StemFit 10 μM ROCK-i. Statistical Student's t-test unless otherwise noted.” excluded from section “2.3 formation, expression analysis”. To fix this, above quoted will be included This mistake made during proofing stage now corrected. Non-thermal atmospheric-pressure potentiates mesodermal human induced pluripotent stem cellsKobayashi et al.HeliyonNovember 30, 2022In BriefAtmospheric-pressure plasma; iPS cells; differentiation; DNA damage; Mesoderm. Full-Text PDF Open Access